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Abstract
ABSTRACT
Recently, some research showed that there is an immunomodulator effect in the
fermentation of Rosella kombucha mushroom tea and it can be caused by flavonoid and organic
acid. The purpose of this research was to show the activity of immunomodulator Rosella
kombucha mushroom tea to against the proliferation of lymphocyte cells of Balb/C mice in vitro
and to show the dose and length of fermentation that induces highest immunomodulator activity. Â
Kombucha mushroom was fermented for 5 days, 10 days and also 15 days with boiled
Rosella tea, water and sugar. The yield of fermentation in the concentration of 6,25; 12,5; 25; 50
and 100 µg/mL were tested on lymphocyte cells proliferation by the MTT Assay. The OD (Optical
Density) data obtained was statistically analyzed by nonparametric Friedman test and
continuoused by Mann-Whitney test.
The result of this research showed that the fermentation of Rosella kombucha mushroom
tea has an immunostimulatory activity on lymphocyte cells proliferation with the concentration
6,25; 12,5 and 25 µg/mL in the 10
days and the highest immunostimulatory activity showed by the
concentration 25 µg/mL and 10
days of fermentation time.
Key words: immunomodulatory, optical density, Rosella kombucha mushroom tea.
Recently, some research showed that there is an immunomodulator effect in the
fermentation of Rosella kombucha mushroom tea and it can be caused by flavonoid and organic
acid. The purpose of this research was to show the activity of immunomodulator Rosella
kombucha mushroom tea to against the proliferation of lymphocyte cells of Balb/C mice in vitro
and to show the dose and length of fermentation that induces highest immunomodulator activity. Â
Kombucha mushroom was fermented for 5 days, 10 days and also 15 days with boiled
Rosella tea, water and sugar. The yield of fermentation in the concentration of 6,25; 12,5; 25; 50
and 100 µg/mL were tested on lymphocyte cells proliferation by the MTT Assay. The OD (Optical
Density) data obtained was statistically analyzed by nonparametric Friedman test and
continuoused by Mann-Whitney test.
The result of this research showed that the fermentation of Rosella kombucha mushroom
tea has an immunostimulatory activity on lymphocyte cells proliferation with the concentration
6,25; 12,5 and 25 µg/mL in the 10
days and the highest immunostimulatory activity showed by the
concentration 25 µg/mL and 10
days of fermentation time.
Key words: immunomodulatory, optical density, Rosella kombucha mushroom tea.