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Submitted
June 24, 2016
Published
June 1, 2016
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Abstract
ABSTRACT
People on Timor island use Faloak (Sterculia quadrifida R.Br.) bark as herbal remedy to
cure diseases such as liver diseases, gastroenteritis and as stamina booster. This study aims to
analyze the ability of anticancer fraction of faloak bark ethanol extract. Â
The mashed bark of faloak plants extracted by maceration method using ethanol. The
ethanol extract fractionated using preparative thin layer chromatography with mixture mobile
phase which, chloroform, n-butanol, ethyl acetate with ratio (3: 4: 1,5). Chromatogram detected
with visible light, UV254, UV366, and cerium sulfate reagent, then marked. Fraction obtained five
fractions. Cytotoxic test against T47D cell and Vero cell conducted using MTT, IC50 and
selectivity sndex (SI) used as an anticancer efficacy parameter.
Fraction 4 showed moderate anticancer activity with IC50 of 21.89 μg/mL in T47D cell
line.
Keywords: Faloak bark, anticancer activiy, T47D cell line
People on Timor island use Faloak (Sterculia quadrifida R.Br.) bark as herbal remedy to
cure diseases such as liver diseases, gastroenteritis and as stamina booster. This study aims to
analyze the ability of anticancer fraction of faloak bark ethanol extract. Â
The mashed bark of faloak plants extracted by maceration method using ethanol. The
ethanol extract fractionated using preparative thin layer chromatography with mixture mobile
phase which, chloroform, n-butanol, ethyl acetate with ratio (3: 4: 1,5). Chromatogram detected
with visible light, UV254, UV366, and cerium sulfate reagent, then marked. Fraction obtained five
fractions. Cytotoxic test against T47D cell and Vero cell conducted using MTT, IC50 and
selectivity sndex (SI) used as an anticancer efficacy parameter.
Fraction 4 showed moderate anticancer activity with IC50 of 21.89 μg/mL in T47D cell
line.
Keywords: Faloak bark, anticancer activiy, T47D cell line