Article Sidebar

Submitted
September 16, 2023
Published
December 29, 2024
Download
PDF
Statistic
Read Counter : 15 Download : 15

Main Article Content

Abstract

Kejek tea (Camellia sinensis L. Kuntze) is a green tea with high antioxidant content so that it has potentially cytotoxic activity. One of the toxicity tests of an extract is BSLT test using Artemia which are determined based on the number of larvae death. The purpose of this study was to determine the cytotoxic potential of kejek tea infusion against Artemia franciscana Kellogg larvae using the Brine Shrimp Lethality Test. This study was an experimental study using 150 larvae of Artemia with the test concentration divided into 5 groups, 1 negative control and 4 series of infusion concentrations, each concentration containing 10 larvae with three replications. The infusion concentrations used were 1000, 500, 100 and 50 ppm, respectively. Observations were determined on larval mortality 24 hours after infusion. The results of the probit analysis showed that the LC50 of infusion of Kejek tea leaves was 6.1317 ppm. This indicated that the infusion of kejek tea leaves had cytotoxic potential against Artemia larvae according to the BSLT method with a LC50 of < 30 ppm.

Keywords

Brine shrimp BSLT Camellia sinensis sitotoksik teh kejek

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Author Biography

Dani Sujana, STIKes Karsa Husada Garut

Program Studi Diploma III Farmasi, STIKes Karsa Husada Garut

References

  1. Aldizal, R., Rizkio, M., Rustamsyah, A., Fadhlillah, F.M., Perdana, F., Inayah, A.A, 2021. The Effect Of Processing Methods On Characteristic Phenolic Content, Flavonoid Content, and Antioxidant Activity of Garut Traditional Tea (Kejek Tea). Jurnal ilmiah Farmako Bahari 12, 69–79.
  2. Budiman, F.A., Hidayat, F., 2021. Uji Toksisitas Akut Ekstrak Etanol Umbi Bit (Beta Vulgaris L.) Dengan Metode Bslt (Brine Shrimp Lethality Test). Jurnal Health Sains 2, 310–315. https://doi.org/10.46799/jhs.v2i3.129
  3. Direktorat Jendral Pengawasan Obat dan Makanan (Ditjen POM). 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Cetakan Pertama. Departemen Kesehatan RI. Jakarta.
  4. Dzakiyyah, M., Rustamsyah, A., Aldizal Mahendra Rizkio Syamsudin, R., Sujana, D., 2023. Review : Aktifitas Farmakologi Dan Studi Fitokimia Teh Hitam Indonesia (Camellia sinensis (L.) Kuntze), Medical Sains : Jurnal Ilmiah Kefarmasian.
  5. FAO, 2020. Top 10 Country Production of Tea leaves 2020. https://www.fao.org/faostat/en/#rankings/countries_by_commodity.
  7. Febrianti, D.R., Mahrita, M., Ariani, N., Putra, A.M.P., Noorcahyati, N., 2019. Uji Kadar Sari Larut Air Dan Kadar Sari Larut Etanol Daun Kumpai Mahung (Eupathorium inulifolium H.B.&K). Jurnal Pharmascience 6, 19. https://doi.org/10.20527/jps.v6i2.7346
  8. Flieger, J., Flieger, W., Baj, J., Maciejewski, R., 2021. Antioxidants: Classification, natural sources, activity/capacity measurements, and usefulness for the synthesis of nanoparticles. Materials 14. https://doi.org/10.3390/ma14154135
  9. Gusungi, D.E., Maarisit, W., Hariyadi, H., Potalangi, N.O., 2020. Studi Aktivitas Antioksidan Dan Antikanker Payudara (MCF-7) Ekstrak Etanol Daun Benalu Langsat Dendrophthoe pentandra. Biofarmasetikal Tropis 3, 166–174. https://doi.org/10.55724/j.biofar.trop.v3i1.274
  11. Hartati, F.K., 2020. Baun dan Pelepah Talas, Kandungan Nutrisi, Fitokimia, Antioksidan dan Uji Toksisitas.
  12. Haryoto, Muhtadi, Indrayudha, P., Azizah, T., Suhendi, A., Haryoto, Muhtadi, Peni Indrayudha, Tanti Azizah, A.S., 2013. Aktivitas Sitotoksik Ekstrak Etanol Tumbuhan Sala (Cynometra ramiflora Linn) Terhadap Sel HeLa, T47D dan WiDR. Jurnal Penelitian Saintek 18, 21–28.
  13. Indonesia Invesment, 2015. Teh Indonesia. Retrieved September 15, 2023, https://www.indonesia-investments.com/id/bisnis/komoditas/teh/item240
  14. Kemenkes RI, 2017. Farmakope Herbal Indonesia. Kementrian Kesehatan Republik Indonesia Edisi II, 213–218.
  15. Kopustinskiene, D.M., Jakstas, V., Savickas, A., Bernatoniene, J., 2020. Flavonoids as anticancer agents. Nutrients. https://doi.org/10.3390/nu12020457
  16. Panche, A.N., Diwan, A.D., Chandra, S.R., 2016. Flavonoids: An overview. J Nutr Sci. https://doi.org/10.1017/jns.2016.41
  17. Redjeki, S., 2015. Uji Aktivitas Antimikroba Infusum Teh Hijau Dan Teh Hitam (Camellia sinensis (L.) Kuntze) Terhadap Escherichia coli Dan Candida albicans. Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi 11, 104. https://doi.org/10.36465/jkbth.v11i1.50
  18. Sujana, D. Wardani, D., Nurul, N., 2020. Review Artikel : Potensi Likopen Dari Buah Tomat (Solanum lycopersicum L) Sebagai Antiaging Topikal. Jurnal Insan Farmasi Indonesia 3, 56–65. https://doi.org/10.36387/jifi.v3i1.479
  19. Sari, I., Miranda, T., Sadli, 2016. The Cytotoxic Activity Of N-Hexane The Cytotoxic Activity Of N-Hexane Extract Of Kersen(Muntingia Calabura Linn.) Extract Of Kersen(Muntingia Calabura Linn.) Leaves Using The Brine Shrimp Lethality Leaves Using The Brine Shrimp Lethality Test (BSLT) Method. jurnal natural 16, 1631–1638.
  20. Vrancheva, R., Ivanov, I., Dincheva, I., Badjakov, I., Pavlov, A., 2021. Triterpenoids and other non-polar compounds in leaves of wild and cultivated vaccinium species. Plants 10, 1–16. https://doi.org/10.3390/plants10010094
  21. Wang, Q., Xie, C., Xi, S., Qian, F., Peng, X., Huang, J., Tang, F., 2020. Radioprotective effect of flavonoids on ionizing radiation-induced brain damage. Molecules. https://doi.org/10.3390/molecules25235719