Main Article Content
Abstract
This study evaluated the profile of microbial growth inhibition on tofu treated by the essential oil of the selected spices. The microbial growth on the tofu was visually observed by the pour plate method, while their number was enumerated by the indirect optical density (OD) method. All the tested essential oils were visually reduced the microbial growth on tofu during storage. Each spices oil showed a different inhibition pattern, with bay leaf, galangal, and ginger oils exerting favorable effects for the most prolonged period. The day-bay-day comparison demonstrated that the best inhibition effect was on day-6 and -8, with bay leaf and galangal oils showing the most promising activity. The best profile of both essential oils might play a vital role in the tofu preservation process by natural products.
Article Details
References
- Al-Dhahli, A. S., Al-Hassani, F. A., Alarjani, K. M., Yehia, H. M., Al-Lawati, W. M., Azmi, S. N. H., & Khan, S. A. (2020). Essential oil from the rhizomes of the Saudi and Chinese Zingiber officinale cultivars: Comparison of chemical composition, antibacterial and molecular docking studies. Journal of King Saud University – Science, 32, 3343–3350. https://doi.org/10.1016/j.jksus.2020.09.020
- Ananchaipattana, C., Hosotani, Y., Kawasaki, S., Pongswat, S., Latiful, B. M., Isobe, S., & Inatsu, Y. (2012). Bacterial contamination of soybean curd (tofu) sold in Thailand. Food Science and Technology Research, 18(6), 843–848. https://doi.org/10.3136/fstr.18.843
- Ari Apriliani, Sukarsa, & Hidayah, H. A. (2014). Kajian etnobotani tumbuhan sebagai bahan tambahan pangan secara tradisional oleh masyarakat di Kecamatan Pekuncen Kabupaten Banyumas. Scripta Biologica, 1(1), 76–84.
- Bassolé, I. H. N., Lamien-Meda, A., Bayala, B., Obame, L. C., Ilboudo, A. J., Franz, C., … Dicko, M. H. (2011). Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination. Phytomedicine, 18(12), 1070–1074. https://doi.org/10.1016/J.PHYMED.2011.05.009
- Carovic´-Stanko, K., Orlic´, S., Politeo, O., Strikic´, F., Kolak, I., Milos, M., & Satovic, Z. (2010). Composition and antibacterial activities of essential oils of seven Ocimum taxa. Food Chemistry, 119(1), 196–201. https://doi.org/10.1016/j.foodchem.2009.06.010
- Hamad, A., Alifah, A., Permadi, A., & Hartanti, D. (2016). Chemical constituents and antibacterial activities of crude extract and essential oils of Alpinia galanga and Zingiber officinale. International Food Research Journal, 23(2), 837–841.
- Hamad, A., Ramadhan, M. B., Dewi, D. Y. S., Djalil, A. D., & Hartanti, D. (2020). Preservation potential of lemon basil essential oil on tofu: Development of a natural food preservative. IOP Conference Series: Materials Science and Engineering, 771, article ID 012037. https://doi.org/10.1088/1757-899X/771/1/012037
- Hamad, Alwani, Djalil, A. D., Saputri, E. Y., Yulianingsih, N., & Hartanti, D. (2020). Bay leaf essential oils inhibited microbial growth and exerted potential preservation effects on tofu. Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering, 3(2), 46–52. https://doi.org/10.21776/ub.afssaae.2020.003.02.2
- Hamad, Alwani, Mahardika, M. G. P., Istifah, & Hartanti, D. (2016). Antimicrobial and volatile compounds study of four spices commonly used in Indonesian culinary. Journal of Food and Pharmaceutical Sciences, 4(1), 1–5.
- Hamad, Alwani, Mahardika, M. G. P., Yuliani, I., & Hartanti, D. (2017). Chemical constituents and antimicrobial activities of essential oils of Syzygium polyanthum and Syzygium aromaticum. Rasayan Journal of Chemistry, 10(2), 564–569. https://doi.org/10.7324/RJC.2017.102169
- Hamad, Alwani, Mentari, M., Djalil, A. D., & Hartanti, D. (2016). Chemical constituents of ginger (Zingiber officinale Roscoe) essential oil and its potency as natural preservative on fresh chicken meat. In I. Epriliati, I. W. Anna, Y. Marsono, S. R. Endang, E. T. Otunola, P. A. Sopade, … E. Chukeatirote (Eds.), Proceeding of International Food Conference 2016: Innovation of Foods Technology to Improve Food Security and Health. Surabaya: Universitas Katolik Widya Mandala Surabaya.
- Hamad, Alwani, Nuritasari, A., & Hartanti, D. (2017). Chemical composition and antimicrobial study of essential oil of lemongrass (Cymbopogon citratus). Der Pharmacia Letter, 9(5), 109–116.
- Hamad, Alwani, Nurlaeli, E., Pradani, D. Y., Djalil, A. D., & Hartanti, D. (2019). Application of lemongrass as natural preservatives for tofu. Jurnal Teknologi Dan Industri Pangan, 30(2), 100–109. https://doi.org/10.6066/jtip.2019.30.2.100
- Hamad, Alwani, Saputri, E. Y., & Hartanti, D. (2016). The use of bay-leaf essential oil as raw chicken meat’s natural preservative. The 2nd International Conference on Food Agriculture and Natural Resources, 1–6. Malang: Universitas Brawijaya.
- Hartanti, D., Djalil, A. D., Yulianingsih, N., & Hamad, A. (2019). The effect of infusion of Syzygium polyanthum (Wight) Walp. leaves as natural preservative chicken meats. Jurnal Kefarmasian Indonesia, 9(1), 19–27. https://doi.org/10.22435/jki.v9i1.1100
- Lee, D. Y., Kwon, K. H., Chai, C., & Oh, S. W. (2017). Microbial contamination of tofu in Korea and growth characteristics of Bacillus cereus isolates in tofu. LWT, 78, 63–69. https://doi.org/10.1016/j.lwt.2016.11.081
- Liu, Q., Meng, X., Li, Y., Zhao, C.-N., Tang, G.-Y., & Li, H.-B. (2017). Antibacterial and antifungal activities of spices. International Journal of Molecular Sciences, 18(6), article ID 1283. https://doi.org/10.3390/ijms18061283
- Monteiro, P. C., Majolo, C., Chaves, F. C. M., Bizzo, H. R., O’Sullivan, F. L. A., & Chagas, E. C. (2021). Antimicrobial activity of essential oils from Lippia sidoides, Ocimum gratissimum and Zingiber officinale against Aeromonas spp. Journal of Essential Oil Research, 33(2), 152–161. https://doi.org/10.1080/10412905.2020.1848653
- Ribeiro, T. T. B. C., Costa, G., & Costa, M. da. (2017). Microbial contamination in industrial tofu. Ciência Rural, 47(03), article ID e20160234. https://doi.org/10.1590/0103-8478cr20160234
- Tofiño-Rivera, A., Ortega-Cuadros, M., Galvis-Pareja, D., Jiménez-Rios, H., Merini, L. J., & MartÃnez-Pabón, M. C. (2016). Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells. Journal of Ethnopharmacology, 194, 749–754. https://doi.org/10.1016/j.jep.2016.10.044
- Umaru, I. J., Umaru, K. I., & Umaru, H. A. (2020). Phytochemical screening, isolation, characterization of bioactive and biological activity of bungkang, (Syzygium polyanthum) root-bark essential oil. Korean Journal of Food & Health Convergence, 6(3), 5–21. https://doi.org/10.13106/kjfhc.2020.vol6.no3.5.
- Zhou, C., Li, C., Siva, S., Cui, H., & Lin, L. (2021). Chemical composition, antibacterial activity and study of the interaction mechanisms of the main compounds present in the Alpinia galanga rhizomes essential oil. Industrial Crops and Products, 165, article ID 113441. https://doi.org/10.1016/j.indcrop.2021.113441
References
Al-Dhahli, A. S., Al-Hassani, F. A., Alarjani, K. M., Yehia, H. M., Al-Lawati, W. M., Azmi, S. N. H., & Khan, S. A. (2020). Essential oil from the rhizomes of the Saudi and Chinese Zingiber officinale cultivars: Comparison of chemical composition, antibacterial and molecular docking studies. Journal of King Saud University – Science, 32, 3343–3350. https://doi.org/10.1016/j.jksus.2020.09.020
Ananchaipattana, C., Hosotani, Y., Kawasaki, S., Pongswat, S., Latiful, B. M., Isobe, S., & Inatsu, Y. (2012). Bacterial contamination of soybean curd (tofu) sold in Thailand. Food Science and Technology Research, 18(6), 843–848. https://doi.org/10.3136/fstr.18.843
Ari Apriliani, Sukarsa, & Hidayah, H. A. (2014). Kajian etnobotani tumbuhan sebagai bahan tambahan pangan secara tradisional oleh masyarakat di Kecamatan Pekuncen Kabupaten Banyumas. Scripta Biologica, 1(1), 76–84.
Bassolé, I. H. N., Lamien-Meda, A., Bayala, B., Obame, L. C., Ilboudo, A. J., Franz, C., … Dicko, M. H. (2011). Chemical composition and antimicrobial activity of Cymbopogon citratus and Cymbopogon giganteus essential oils alone and in combination. Phytomedicine, 18(12), 1070–1074. https://doi.org/10.1016/J.PHYMED.2011.05.009
Carovic´-Stanko, K., Orlic´, S., Politeo, O., Strikic´, F., Kolak, I., Milos, M., & Satovic, Z. (2010). Composition and antibacterial activities of essential oils of seven Ocimum taxa. Food Chemistry, 119(1), 196–201. https://doi.org/10.1016/j.foodchem.2009.06.010
Hamad, A., Alifah, A., Permadi, A., & Hartanti, D. (2016). Chemical constituents and antibacterial activities of crude extract and essential oils of Alpinia galanga and Zingiber officinale. International Food Research Journal, 23(2), 837–841.
Hamad, A., Ramadhan, M. B., Dewi, D. Y. S., Djalil, A. D., & Hartanti, D. (2020). Preservation potential of lemon basil essential oil on tofu: Development of a natural food preservative. IOP Conference Series: Materials Science and Engineering, 771, article ID 012037. https://doi.org/10.1088/1757-899X/771/1/012037
Hamad, Alwani, Djalil, A. D., Saputri, E. Y., Yulianingsih, N., & Hartanti, D. (2020). Bay leaf essential oils inhibited microbial growth and exerted potential preservation effects on tofu. Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering, 3(2), 46–52. https://doi.org/10.21776/ub.afssaae.2020.003.02.2
Hamad, Alwani, Mahardika, M. G. P., Istifah, & Hartanti, D. (2016). Antimicrobial and volatile compounds study of four spices commonly used in Indonesian culinary. Journal of Food and Pharmaceutical Sciences, 4(1), 1–5.
Hamad, Alwani, Mahardika, M. G. P., Yuliani, I., & Hartanti, D. (2017). Chemical constituents and antimicrobial activities of essential oils of Syzygium polyanthum and Syzygium aromaticum. Rasayan Journal of Chemistry, 10(2), 564–569. https://doi.org/10.7324/RJC.2017.102169
Hamad, Alwani, Mentari, M., Djalil, A. D., & Hartanti, D. (2016). Chemical constituents of ginger (Zingiber officinale Roscoe) essential oil and its potency as natural preservative on fresh chicken meat. In I. Epriliati, I. W. Anna, Y. Marsono, S. R. Endang, E. T. Otunola, P. A. Sopade, … E. Chukeatirote (Eds.), Proceeding of International Food Conference 2016: Innovation of Foods Technology to Improve Food Security and Health. Surabaya: Universitas Katolik Widya Mandala Surabaya.
Hamad, Alwani, Nuritasari, A., & Hartanti, D. (2017). Chemical composition and antimicrobial study of essential oil of lemongrass (Cymbopogon citratus). Der Pharmacia Letter, 9(5), 109–116.
Hamad, Alwani, Nurlaeli, E., Pradani, D. Y., Djalil, A. D., & Hartanti, D. (2019). Application of lemongrass as natural preservatives for tofu. Jurnal Teknologi Dan Industri Pangan, 30(2), 100–109. https://doi.org/10.6066/jtip.2019.30.2.100
Hamad, Alwani, Saputri, E. Y., & Hartanti, D. (2016). The use of bay-leaf essential oil as raw chicken meat’s natural preservative. The 2nd International Conference on Food Agriculture and Natural Resources, 1–6. Malang: Universitas Brawijaya.
Hartanti, D., Djalil, A. D., Yulianingsih, N., & Hamad, A. (2019). The effect of infusion of Syzygium polyanthum (Wight) Walp. leaves as natural preservative chicken meats. Jurnal Kefarmasian Indonesia, 9(1), 19–27. https://doi.org/10.22435/jki.v9i1.1100
Lee, D. Y., Kwon, K. H., Chai, C., & Oh, S. W. (2017). Microbial contamination of tofu in Korea and growth characteristics of Bacillus cereus isolates in tofu. LWT, 78, 63–69. https://doi.org/10.1016/j.lwt.2016.11.081
Liu, Q., Meng, X., Li, Y., Zhao, C.-N., Tang, G.-Y., & Li, H.-B. (2017). Antibacterial and antifungal activities of spices. International Journal of Molecular Sciences, 18(6), article ID 1283. https://doi.org/10.3390/ijms18061283
Monteiro, P. C., Majolo, C., Chaves, F. C. M., Bizzo, H. R., O’Sullivan, F. L. A., & Chagas, E. C. (2021). Antimicrobial activity of essential oils from Lippia sidoides, Ocimum gratissimum and Zingiber officinale against Aeromonas spp. Journal of Essential Oil Research, 33(2), 152–161. https://doi.org/10.1080/10412905.2020.1848653
Ribeiro, T. T. B. C., Costa, G., & Costa, M. da. (2017). Microbial contamination in industrial tofu. Ciência Rural, 47(03), article ID e20160234. https://doi.org/10.1590/0103-8478cr20160234
Tofiño-Rivera, A., Ortega-Cuadros, M., Galvis-Pareja, D., Jiménez-Rios, H., Merini, L. J., & MartÃnez-Pabón, M. C. (2016). Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells. Journal of Ethnopharmacology, 194, 749–754. https://doi.org/10.1016/j.jep.2016.10.044
Umaru, I. J., Umaru, K. I., & Umaru, H. A. (2020). Phytochemical screening, isolation, characterization of bioactive and biological activity of bungkang, (Syzygium polyanthum) root-bark essential oil. Korean Journal of Food & Health Convergence, 6(3), 5–21. https://doi.org/10.13106/kjfhc.2020.vol6.no3.5.
Zhou, C., Li, C., Siva, S., Cui, H., & Lin, L. (2021). Chemical composition, antibacterial activity and study of the interaction mechanisms of the main compounds present in the Alpinia galanga rhizomes essential oil. Industrial Crops and Products, 165, article ID 113441. https://doi.org/10.1016/j.indcrop.2021.113441