OPTIMASI CAIRAN PENYARI PADA PEMBUATAN EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifous Roxb) SECARA MASERASI TERHADAP KADAR FENOLIK DAN FLAVONOID TOTAL.
DOI:
https://doi.org/10.36499/jim.v6i2.118Abstract
Pandan wangi (Pandanus amaryllifous Roxb) merupakan salah satu tanaman yang digunakan sebagai tonikum, penambah nafsu makan, penenang, penyedap, pewangi dan pemberi warna hijau pada masakan. Mengandung alkaloida, saponin, flavonoida, tanin, polifenol, fenil propanoid, dan zat warna (Dalimartha, 2000:103). Penelitian ini bertujuan untuk mengetahui kadar fenolik dan flavonoid total dari hasil ekstraksi cairan penyari yang berbeda serta mengetahui cairan penyari yang paling optimal menarik senyawa fenolik dan flavonoid. Penyarian dilakukan dengan cara maserasi air; etanol 96%; dan air : etanol 96% (0,5 : 0,5). Uji pendahuluan kandungan kimianya menggunakan reaksi warna dan KLT. Hasil KLT senyawa flavonoid dari ketiga penyari adalah sama yaitu Rf = 0,60. Optimasi cairan penyari menggunakan metode simplex lattice design diperoleh dengan uji t. Hasil kadar fenolik total ekstrak daun pandan wangi penyari air = 24,0004 mg/g ekstrak; etanol 96% = 478,7629 mg/g ekstrak; air : etanol 96% (0,5 : 0,5) = 308,9702 mg/g ekstrak. Kadar flavonoid total ekstrak daun pandan wangi penyari air = 4,6102 mg/g ekstrak; etanol 96% = 99,4086 mg/g ekstrak; air : etanol 96% (0,5 : 0,5) = 33,6216 mg/g ekstrak. Hasil optimasi menunjukkan cairan penyari yang paling maksimal menarik senyawa fenolik dan flavonoid adalah etanol 96% dengan kadar fenolik total 478,7629 mg/g dan kadar flavonoid total 99,4086 mg/g. Uji t menunjukkan tidak ada perbedaan signifikan kadar fenolik dan flavonoid total antara teori dengan percobaan. Kata kunci : Daun pandan wangi, cairan penyari, kadar fenolik total, kadar flavonoid total, optimasi.Downloads
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