Main Article Content
Abstract
Jatropha curcas L is a plant species that can be used for curing, since it
contains flavonoid, saponin, and tanin. Jatropha multifida L is proved to have
antibacterial activities (Sisunandar, et.al., 2002). This research was intended to
find out the antibacterial activities of ethanol extracts of Jatropha curcas L leaves
on bacteria Staphylococus aureus, Escherichia coli, and Salmonella typhi, find out
the effectiveness of those antibacterial activities and identify the compound
groups contained in the ethanol extracts, the result of antibacterial activity of
ethanol extracts of Jatropha curcas L was analized by statistic.
Employing a maceration method, Jatropha curcas L leaves were extracted
using ethanol 70% for five days. Employing an agar-diffusion method aided by
disk papers and in order to find out the inhibitory area diameters of the extracts,
the extracts were then tested for their antibacterial activities. Qualitative analysis
on the chemical contents of the extracts were conducted employing. TLC method
aided by silica-gel stationary phases for saponin and tanin and cellulose phases for
flavonoid. The motion phases used ethyl acetat-acidformiat-acid acetat-water for
flavonoid, chloroform-methanol for saponin and buthanol-acid acetat-water for
tanin. The result of the test on antibacterial activities were analyzed statistically
using Kruskall Wallis and Mann & Whitney tests.
The analysis showed that ethanol extracts of the Jatropha curcas L leaves
inhibited the growth of Staphylococcus aureus but not the growth of Eschericia
coli and Salmonella typhi. The testing concentrations of 20%, 40%, 60%, 80%,
and 100% b/v produed inhibitory area diameters averaging 8.25, 9.25, 11.00,
13.25, and 19.00 mm respectively. The positive control of Ampicillin produced an
average inhibitory area diameters of 40.00 mm while the solvent control did not
produce any inhibitory area diameters. Statistical test showed that there were
significant differences among the different concentrations of the extracts.Thin Layer Chromatography tests produced yellow color showing the existence of
flavonoid, bluish violet color showing the existence of saponin, and grayish green
color showing the existence of tanin.
Keyword : ethanol extracts of Jatropha curcas L leaves, inhibitory area
diameters, Staphylococcus aureus, Escherichia coli, and Salmonella
typhi
contains flavonoid, saponin, and tanin. Jatropha multifida L is proved to have
antibacterial activities (Sisunandar, et.al., 2002). This research was intended to
find out the antibacterial activities of ethanol extracts of Jatropha curcas L leaves
on bacteria Staphylococus aureus, Escherichia coli, and Salmonella typhi, find out
the effectiveness of those antibacterial activities and identify the compound
groups contained in the ethanol extracts, the result of antibacterial activity of
ethanol extracts of Jatropha curcas L was analized by statistic.
Employing a maceration method, Jatropha curcas L leaves were extracted
using ethanol 70% for five days. Employing an agar-diffusion method aided by
disk papers and in order to find out the inhibitory area diameters of the extracts,
the extracts were then tested for their antibacterial activities. Qualitative analysis
on the chemical contents of the extracts were conducted employing. TLC method
aided by silica-gel stationary phases for saponin and tanin and cellulose phases for
flavonoid. The motion phases used ethyl acetat-acidformiat-acid acetat-water for
flavonoid, chloroform-methanol for saponin and buthanol-acid acetat-water for
tanin. The result of the test on antibacterial activities were analyzed statistically
using Kruskall Wallis and Mann & Whitney tests.
The analysis showed that ethanol extracts of the Jatropha curcas L leaves
inhibited the growth of Staphylococcus aureus but not the growth of Eschericia
coli and Salmonella typhi. The testing concentrations of 20%, 40%, 60%, 80%,
and 100% b/v produed inhibitory area diameters averaging 8.25, 9.25, 11.00,
13.25, and 19.00 mm respectively. The positive control of Ampicillin produced an
average inhibitory area diameters of 40.00 mm while the solvent control did not
produce any inhibitory area diameters. Statistical test showed that there were
significant differences among the different concentrations of the extracts.Thin Layer Chromatography tests produced yellow color showing the existence of
flavonoid, bluish violet color showing the existence of saponin, and grayish green
color showing the existence of tanin.
Keyword : ethanol extracts of Jatropha curcas L leaves, inhibitory area
diameters, Staphylococcus aureus, Escherichia coli, and Salmonella
typhi